Table 1. Oligonucleotides used in this study.

Construct	Primer
EcFbFP_pTrcHis2C	FW: 5′- ATGCCCATGGCGTCGTTCCAGTCGTTCGGC -3′
	RV: 5′- CGATGAATTCTTACTCGAGCAGCTTTTCAT -3′
EcFbFP_ L65F	FW: 5′- GCGCGCTTCTTCCAGGGGAAG -3′
	RV: 5′- CTTCCCCTGGAAGAAGCGCGC -3′
EcFbFP_ R79L	FW: 5′- GACAACATCCTCACCGCGCTG -3′
	RV: 5′- CAGCGCGGTGAGGATGTTGTC -3′
EcFbFP_ N104I	FW: 5′- TGTTCTGGATCGAACTGAAC -3′
	RV: 5′- GTTCAGTTCGATCCAGAACA -3′
EcFbFP_ N104S	FW: 5′- TGTTCTGGAGCGAACTGAAC -3′
	RV: 5′- GTTCAGTTCGCTCCAGAACA -3′