. Author manuscript; available in PMC: 2015 Jun 1.

Published in final edited form as: Ticks Tick Borne Dis. 2014 Mar 12;5(4):401–403. doi: 10.1016/j.ttbdis.2014.01.007

Table 1.

Primers used for PCR amplification and sequencing.

Gene	Primer name	Sequence	Expected product size (bp)

16S rRNA	T50^a	GTTACGACTTCACCCTCCT	1488
	FD3^a	AGAGTTTGATCCTGGCTTAG
	Rec 4^a	ATGCTAGAAACTGCATGA
	Rec 9^a	TCGTCTGAGTCCCCATCT
	16RnaL^b	CTGGCAGTGCGTCTTAAGCA
	16RnaR^b	GTATTCACCGTATCATTCTGATATAC
	16S−2^a	TACAGGTGCTGCATGGTTGTCG
	16S−1^a	TAGAAGTTCGCCTTCGCCTCTG

glpQ	glpQF+1^a	GGGGTTCTGTTACTGCTAGTGCCATTAC	1002
	Rev−2^a	CAATACTAAGACCAGTTGCTCCTCCGCC
	glpQF−1^a	CAATTTTAGATATGTCTTTACCTTGTTGTTTATGCC
	Rev−1^a	GCACAGGTAGGAATGTTGGAATTTATCCTG

flaB	Fla ans 5′^a	TGTGATATCCTTTTAAAGAGACAAATGG	1284
	Fla alt 3′^a	TCTAAGCAATGACAATACATATTGAGG
	flaLL^b	ACATATTCAGATGCAGACAGAGGT
	flaRL^b	GCAATCATAGCCATTGCAGATTGT
	flaLS^b	AACAGCTGAAGAGCTTGGAATG
	flaRS^b	CTTTGATCACTTATCATTCTAATAGC

hpt	hptdegF^c	GCAGAYATTACAAGAGARATGG	433
hpt	hptdegR^c	CYTCRTCACCCCATTGAGTTCC	433

Tick 16S rRNA	Tick16S+1^d	CTGCTCAATGATTTTTTAAATTGCTGTGG	480
	Tick16S−1^d	CCGGTCTGAACTCAGATCAAGT
	Tick16S+2^d	TTGGGCAAGAAGACCCTATGAA
	Tick16S−2^d	TTACGCTGTTATCCCTAGAG
	Tick16S+3^d	ATACTCTAGGGATAACAGCGT
	Tick16S−3^d	AAATTCATAGGGTCTTCTTGTC

Tick 12S rRNA	SR-J-14199^e	TACTATGTTACGACTTAT	430
Tick 12S rRNA	SR-N-14594^e	AAACTAGGATTAGATACCC	430

Amplification primers in bold; all primers used for sequencing.

this study.