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. 2014 Jun 2;9(6):e97379. doi: 10.1371/journal.pone.0097379

Table 1. Sequencing results.

Direct sequencing Multiple displacement amplification
Total number of reads Run1 16758 46547
Run2 4169 1593
Total Mbases Run1 3853532 12891425
Run2 582005 253376
Average read length Run1 229.95±137.26 276.96±165.51
Run2 139.60±89.85 159.06±111.82
Average read quality Run1 36.18±3.48 35.91±3.52
Run2 31.12±3.41 30.84±3.41
GC content (%) Run1 48.94 46.05
Run2 48.54 46.15
Theoretical E. coli coverage of all processed reads Run1 + Run2 0.96 2.83
Theoretical E. coli coverage of all reads mapped to E. coli Run1 + Run2 0.77 0.07
Actual obtained E. coli coverage Run1 + Run2 0.76 0.05

The table shows the number of total reads, total bases, mean and median read length and mean GC content (%) of processed sequences. The theoretical E. coli coverage was calculated from the total obtained Mbp. In fact, the actual coverage was lower than expected, especially in the case of MDAsample.