Table 1. Sequencing results.
Direct sequencing | Multiple displacement amplification | ||
Total number of reads | Run1 | 16758 | 46547 |
Run2 | 4169 | 1593 | |
Total Mbases | Run1 | 3853532 | 12891425 |
Run2 | 582005 | 253376 | |
Average read length | Run1 | 229.95±137.26 | 276.96±165.51 |
Run2 | 139.60±89.85 | 159.06±111.82 | |
Average read quality | Run1 | 36.18±3.48 | 35.91±3.52 |
Run2 | 31.12±3.41 | 30.84±3.41 | |
GC content (%) | Run1 | 48.94 | 46.05 |
Run2 | 48.54 | 46.15 | |
Theoretical E. coli coverage of all processed reads | Run1 + Run2 | 0.96 | 2.83 |
Theoretical E. coli coverage of all reads mapped to E. coli | Run1 + Run2 | 0.77 | 0.07 |
Actual obtained E. coli coverage | Run1 + Run2 | 0.76 | 0.05 |
The table shows the number of total reads, total bases, mean and median read length and mean GC content (%) of processed sequences. The theoretical E. coli coverage was calculated from the total obtained Mbp. In fact, the actual coverage was lower than expected, especially in the case of MDAsample.