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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Gene. 2014 Mar 25;542(2):221–231. doi: 10.1016/j.gene.2014.03.030

Table 3.

Phenotypic and Genotypic Analysis of Tf1 insertion events under nonselective conditions.

Number of Colonies
NSP SP NSP NS*
Transposed Patch Tf1 Plasmid 5-FOAR G418R G418S G418S/neo+ % Transposition† Genotype
YTS6716 pTS1559-9 250 7 243 2 2.8 Diploid
YTS6932 pHL449-1 250 6 244 7 2.4 Haploid
YTS7084 pTS1559-9 250 4 246 0 1.6 Haploid
YTS7133 pTS1559-9 250 7 243 1 2.8 Haploid

NSP = Nonselective Phase

SP = Selective Phase

NS* = neo+ by southern blot hybridization using an 851bp DIG-labeled neo probe.

†

= Transposition frequency of each patch was determined by calculating the total number of 5-FOAR colonies that were also G418R.