Table 5.
| Insertion Clone/Plasmid | Phenotype | Adjacent genes | Protein function |
|---|---|---|---|
| Gene expression (+/−) | |||
| YTS6932-14 | |||
| − | G418S | 5’-SPCC1223.09 | uricase* |
| + | 3’-SPCC1223.10 (eaf1) | RNA pol II transcription elongation factor | |
| YTS6932-38 | |||
| − | G418S | 5’-SPCC1919.03 (amk2) | AMP-activated protein kinase beta subunit* |
| − | 3’-SPCC1919.04 | Uncharacterized membrane protein | |
| YTS6932-45 | |||
| − | G418S | 5’-SPBC776.12 (hsk1) | Protein kinase |
| − | 3’-SPBC776.13 (cnd1) | Condensin complex subunit 1 | |
| YTS6932-121 | |||
| − | G418S | 5’-SPAC4A8.03 (ptc4) | protein phosphatase |
| − | 3’-SPAC4A8.04 (isp6) | vacuolar serine protease | |
| YTS6932-203 | |||
| − | G418S | 5’-SPAC57A7.05 | conserved hypothetical protein |
| − | 3’-SPAC57A7.04 (pabp) | mRNA export shuttling protein | |
| YTS6932-215 | |||
| − | G418S | 5’-SPAC13A11.02 (erg11) | Probable cytochrome P450 protein |
| − | 3’-SPAC13A11.03 (mcp7) | Required for meiotic recombination | |
| YTS6932-230 | |||
| + | G418S | 5’-SPAC3H5.04 (aar2) | U5 snRNP-associated protein |
| − | 3’-SPAC3H5.03 (pkl1) | kinesin-like protein | |
| YTS6716-2 | |||
| − | G418R | 5’-SPCC74.03 (ssp2) | serine/threonine protein kinase |
| − | 3’-SPCC74.04 | amino acid permease* | |
| YTS6716-68/102 | |||
| − | G418S | 5’-SPBC23G7.09 (mat1-Mc) | Mating-type M-specific polypeptide |
| − | 3’-SPBC23G7.10 | NADH-dependent flavin oxidoreductase* | |
| YTS7084-4 | |||
| + | G418R | 5’-SPBC119.03 | conserved hypothetical protein |
| − | 3’-SPBC119.04 (mei3) | meiosis inducing protein | |
| pTS1559-9 | |||
| − | Plasmid | 5’-SPBPB2B2.09 | 2-dehydropantoate 2-reductase |
| − | 3’-SPBPB2B2.10 | Galactose-1-phosphate uridylyltransferase | |
*
predicted protein function