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. 2014 Jun 2;9(6):e97897. doi: 10.1371/journal.pone.0097897

Figure 5. Oral administration of GSNO abrogates tumor growth in A2780 bearing nude mice.

Figure 5

A. Schematic diagram of experimental design. In brief, A2780 ovarian cancer cell line was injected interperitoneally in nude mice and S-nitrosoglutathione (GSNO) was given orally daily from day 3 at the dose of 1 mg/kg of body weight till the end of the study. Phosphate buffered saline (PBS) was given as vehicle. B. Decreased abdominal circumference of GSNO treated mice compared to vehicle treated groups measured at week 4 (**p<0.01 treated compared to vehicle). C. Decreased excised tumor weights of GSNO treated mice compared to vehicle treated at week 4 (***p<0.001 treated compared to vehicle). Results are shown as mean ± SD of 10–14 individual animals. D. Representative gross morphological picture of tumor mass and tumor associated with ovary of vehicle and GSNO treated mice. E. Measurements of viable tumor size of GSNO vs PBS treated mice, as described in methods (***p<0.001 treated compared to vehicle). F. Count of mitotic cells and G. count of CD31 positive vessels per HPF (x400) in GSNO vs PBS treated mice, as described in methods), counts were performed from 5 fields of 3 different tumors from each group. NS; non-significant treated compared to vehicle.