Table 2. Colony number and mutation rate obtained by error-prone PCR mediated mutagenesis.

DNA	# colonies	# colonies with indicated number of mutations							mutation
input	sequenced	0 mut	1 mut	2 mut	3 mut	4 mut	5 mut	>5 mut	rate
500 ng	116	20	30	28	24	8	6	0	1.5 mut/kb
750 ng	120	41	41	19	9	6	3	0	0.8 mut/kb
pooled	236	61	71	47	33	14	9	0	1.3 mut/kb