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Immunohistochemical analysis of NOS-I and III proteins in P12 and P120 bladder. At P12 NOS-I was abundant in nerves in the urothelium but was also identified in the smooth muscle. In the adult NOS-I was abundant in the smooth muscle as well as the urothelium. NOS-III was localized primarily in the developing basement membrane but was also identifiable in blood vessels at P12 and P120. 100× magnification. Arrows indicate NOS-I and III staining.
