Table 1. The characteristics of the investigated STR loci are shown.
Locus | T M (°C) | Size-range (bp) | Repeat motif | A | HO | HE | P ID | P IDsib |
Hir7 | TD 52-50 | 215–273 | (CT)2 | 25 | 0.69 | 0.94 | 7.07×10−3 | 2.82×10−1 |
Hir24 | TD 56-54 | 194–238 | (AGTG)4 | 12 | 0.46 | 0.88 | 2.06×10−4 | 9.06×10−2 |
Hir10 | TD 52-50 | 147–201 | (GTTT)4 | 13 | 0.75 | 0.85 | 8.79×10−6 | 3.06×10−2 |
Hru5 | TD 52-50 | 110–140 | (GT)9 | 15 | 0.66 | 0.84 | 4.01×10−7 | 1.05×10−2 |
Hir20 | TD 50-48 | 231–275 | (ATAG)9 | 15 | 0.76 | 0.84 | 1.90×10−8 | 3.61×10−3 |
Hir6 | TD 56-52 | 178–218 | (TCTA)11 | 11 | 0.80 | 0.84 | 9.19×10−10 | 1.24×10−3 |
Hir11 | TD 50-48 | 166–214 | (GATA)7 | 12 | 0.78 | 0.83 | 4.71×10−11 | 4.34×10−4 |
Hir4 | TD 56-54 | 259–297 | (GTTT)5 | 16 | 0.39 | 0.79 | 3.22×10−12 | 1.63×10−4 |
Hir5 | TD 50-48 | 213–233 | (GTTT)4 | 6 | 0.45 | 0.71 | 4.48×10−13 | 6.98×10−5 |
Hir15 | TD 50-48 | 209–249 | (ATGT)2 | 9 | 0.55 | 0.47 | 8.16×10−14 | 3.29×10−5 |
T M (°C), annealing temperature; TD, touch-down PCR; A, number of alleles per locus; H O, mean observed heterozygosity; H E, mean expected heterozygosity; P ID, probability that two individuals drawn at random share identical genotypes; P IDsib, probability of identity among siblings. STR loci are sorted according to the increasing order of their P ID (P IDsib) single-locus values (i.e., the locus at the top is the most informative one), and a sequentially multi-loci P ID (P IDsib) is reported for each locus.