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. 2014 Jun 2;9(6):e98751. doi: 10.1371/journal.pone.0098751

Figure 1. Shh and Ihh expression levels along the intestinal rostro-caudal axis and loss of Shh expression in the ileal and colonic epithelium of Shh ΔIEC mice.

Figure 1

Quantification of Ihh and Shh mRNA expression levels along the intestinal rostro-caudal axis was performed by qPCR (A) (n = 7). Total mRNA from intestinal epithelial fractions isolated along the villus-to-crypt axis by the Weiser method (n = 3) were used to monitor mRNA levels of sucrase-isomaltase and Shh along the crypt-to-villus axis (B). Semi-quantitative PCR analysis was performed to detect Shh mRNA expression levels in the ileum and colon of Shh ΔIEC and control mice (C and D). Semi-quantitative PCR analysis showed no modulation in Ihh mRNA expression levels in Shh ΔIEC mice when compared with control animals (E and F). Statistical analysis indicated a significant 1.2-fold decrease in small intestine length of 180-day-old Shh ΔIEC mice when compared to controls (G) whereas no modulation was observed in the colon (H) (n = 7). One-way ANOVA *p<0.05 (B), Student T-test *p<0.05, **p<0.01 (D and G). Error bar represent SEM. SI, sucrase-isomaltase; Duo, duodenum; Jej, jejunum, PC, proximal colon; DC, distal colon.