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. 2014 Jun 2;9(6):e98751. doi: 10.1371/journal.pone.0098751

Figure 3. Loss of intestinal epithelial Shh signaling does not activate the Wnt/β-catenin pathway.

Figure 3

Immunostaining with an anti-β-catenin antibody revealed no modulation of nuclear translocation of β-catenin (black arrows) between controls (A) and mutant mice (B). Immunostaining with an anti-E-cadherin antibody in both control (C) and mutant mice (D) enabled to determine the localization of the cell membrane relative to that of the nucleus. Western blot analysis for c-Myc and cyclin D1/D2 classical targets of epithelial cell proliferation was performed on ileal extracts from control and Shh ΔIEC mice (E). Densitometry analysis of exposed films using ImageJ revealed no significant modulation in c-Myc and cyclin D1/D2 expression levels in Shh ΔIEC mice compared to controls (F) (n = 3) Student t-test. Scale bar: 50 µm.