Table 1. Predicted features of wild type and mutant human DsRed-NT5E fusion proteins.

DsRed-hNT5E protein	Amino acid length§	Isoelectric point§	Molecular weight (in Daltons)§	Peptide signal¶	Thrombin cleavage site¶	C-terminal GPI anchor
Wild type	825/797	6.06/5.95	91360.1/88657.8	1:27	1:273	Yes
F1	471/443	5.61/5.46	51905.0/49202.7	1:27	1.273	No
F2	825/797	6.06/5.95	91420.2/88717.9	1:27	1.273	Yes
F3	793/765	5.94/5.83	87666.7/84964.4	1:27	1:273	No

^§Before/after maturation.

^¶Total number/position of residue(s).

In silico analysis of wild type and mutant DsRed-tagged human NT5E (DsRed-hNT5E) fusion protein sequences was performed using various bioinformatics tools: ExPASy ProtParam tool for amino acid length, isoelectric point, molecular weight [49]; ExPASy SignalP 4.0 tool for peptide signal location identification [50]; ExPASy PeptideCutter tool for thrombin cleavage site identification [49]; and GPI-SOM tool for C-terminal GPI anchor site recognition [51]. Note: number/position of residues may differ from those of native human NT5E protein.