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. 2014 Jun 2;9(6):e98606. doi: 10.1371/journal.pone.0098606

Figure 5. WASH is required for efficient antigen presentation and T cell priming.

Figure 5

(A) BMDCs from WASHf/f LysM-Cre mice and control LysM-Cre mice were cultured with OT-II T cells and ovalbumin-derived peptide antigen at the indicated doses. After three days in culture, T cell proliferation was determined by flow cytometry and cell count. (B) Alternatively, BMDCs were first pulsed with peptide antigen, then washed and cultured at the indicated cell numbers with OT-II T cells. T cell proliferation was determined by flow cytometry and cell count after three days. (C) WASHf/f CD11c-Cre mice and control WASHf/f mice were immunized by subcutaneous injection of ovalbumin peptide in CFA. Seven days later, draining lymph nodes were harvested and restimulated in vitro with ovalbumin peptide. Antigen-specific T cells producing IL-2 upon restimulation were enumerated by ELISPOT.