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. 2014 Jun 2;9(6):e98868. doi: 10.1371/journal.pone.0098868

Figure 3. bcl-2 is a target gene of miR-15a, but not miR-16 in chicken lung.

Figure 3

(A) miR-15a regulates the translation of bcl-2 mRNA through a sequence that is not conserved with human sequence. miR-15a and the binding site in the gga-bcl-2 3′-UTR are shown, but miR-16 shows no target site in this part of the sequence. (B) miR-15a/16 have a consistent target site in human bcl-2 3′-UTR. The miR-15a binding site in the bcl-2 3′-UTR sequence mediates translation repression by miR-15a. (C) The luciferase reporter vector contains two luciferase cDNAs, Renilla luciferase (hRluc) and firefly luciferase (hluc). The bcl-2 3′-UTR was fused to the hRluc cDNA downstream sequence. In co-transfected cells, the miR-15a mimic decreased the expression of hRluc and miR-15a mimic inhibitor rescued hRluc activity; no differences were seen for miR-16. Data are expressed as the mean ± SEM. * P<0.05, ** P<0.01.