Table 3. Production of PHA in P. extremaustralis and recombinants P. putida GPp104.
Strain | Carbon source | Time of culture (h) | PHB content (wt% of CDW) | PHA content (PHHX+ PHO) (wt% of CDW) |
P. extremaustralis 14-3b | 0.25% octanoate | 8 | 13.60±1.40 | 3.40±0.30 |
0.25% octanoate | 24 | 35.80±3.60 | 1.05±0.10 | |
0.27% glucose | 8 | 0.37±0.04 | ND | |
0.27% glucose | 24 | 1.30±0.10 | ND | |
3% glucose | 24 | 12.20±1.00 | 0.26±0.02 | |
P. putida GPp104 pMHZ59 | 0.25% octanoate | 24 | 2.60±0.30 | 28.00±0.80 |
P. putida GPp104 pMMic2 | 0.25% octanoate | 24 | 0.10±0.01 | 9.60±1.00 |
P. putida GPp104 pMHZ59 | 2% glucose | 24 | 0.60±0.01 | 6.10±0.82 |
P. putida GPp104 pMMic2 | 2% glucose | 24 | 0.27±0.03 | 2.66±0.33 |
P. putida GPp104 pBBR1MCS-5 | 0.25% octanoate | 24 | ND | ND |
P. putida GPp104 pBBR1MCS-5 | 2% glucose | 24 | ND | ND |
Cultures were grown in 0.5NE2 with the indicate carbon source.
ND = no detected, PHHX: polyhydroxyhexanoate, PHO: polyhydroxyoctanoate.