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. 2014 Jun 2;9(6):e98873. doi: 10.1371/journal.pone.0098873

Table 3. Production of PHA in P. extremaustralis and recombinants P. putida GPp104.

Strain Carbon source Time of culture (h) PHB content (wt% of CDW) PHA content (PHHX+ PHO) (wt% of CDW)
P. extremaustralis 14-3b 0.25% octanoate 8 13.60±1.40 3.40±0.30
0.25% octanoate 24 35.80±3.60 1.05±0.10
0.27% glucose 8 0.37±0.04 ND
0.27% glucose 24 1.30±0.10 ND
3% glucose 24 12.20±1.00 0.26±0.02
P. putida GPp104 pMHZ59 0.25% octanoate 24 2.60±0.30 28.00±0.80
P. putida GPp104 pMMic2 0.25% octanoate 24 0.10±0.01 9.60±1.00
P. putida GPp104 pMHZ59 2% glucose 24 0.60±0.01 6.10±0.82
P. putida GPp104 pMMic2 2% glucose 24 0.27±0.03 2.66±0.33
P. putida GPp104 pBBR1MCS-5 0.25% octanoate 24 ND ND
P. putida GPp104 pBBR1MCS-5 2% glucose 24 ND ND

Cultures were grown in 0.5NE2 with the indicate carbon source.

ND =  no detected, PHHX: polyhydroxyhexanoate, PHO: polyhydroxyoctanoate.