Skip to main content
. 2014 Jun 2;9(6):e98698. doi: 10.1371/journal.pone.0098698

Figure 6. Association between high canonical Wnt activity and LEF1 expression.

Figure 6

(A) mRNA expression of TCF1, LEF1, TCF3 and TCF4 in two different passages of canine mammary cell lines normalized to the expression of Rps19. (B) β-catenin, LEF1 and Axin2 mRNA expression in CMT-U27 cells that were either non-transfected (control) or transfected with mock control (mock), LEF1 siRNA (LEF1) or β-catenin siRNA (β-catenin). mRNA expression was analyzed 24 h and 48 h post-transfection. (C) Average TOP/FOP ratio in CMT-U27 cells as described in (B). * indicates p<0.05 and **p<0.01 compared to the mock control. (D) Rps19 normalized mRNA expression of LEF1 in a panel of canine mammary tumors (tumor) and normal mammary tissue (normal) from the same dog.