Figure 4. TgCBAP localizes to forming daughter parasites during endodiogeny.

Immunofluorescence localization of endogenously tagged TgCBAP-3xmyc within T. gondii ku80::hxgprt parasites undergoing endodiogeny. Parasites were stained with anti-IMC1 (red) to outline the mother and daughter parasites and anti-myc (green) to localize TgCBAP-3xmyc; deconvolved maximum intensity projections are shown. (A,B) A basal ring of TgCBAP staining is evident in both the mother cell (arrowhead) and forming daughters (arrows). The peripheral puncta are difficult to resolve on the surface of the forming daughter cells; however, 3D reconstructions suggest that these may be present as well (see Movie S1) (C) Peripheral puncta organized in stripes are clearly evident in mature, emerging daughter cells (arrows), as are the basal rings (arrowheads). Scale bars = 5 µm.