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. Author manuscript; available in PMC: 2014 Jun 3.
Published in final edited form as: Sci Signal. 2013 Jun 11;6(279):ra45. doi: 10.1126/scisignal.2004015

Figure 4. AKT regulates lysosomal progression of EGFR.

Figure 4

A. Cells were pre-treated with chloroquine and stimulated with Alexa-488 EGF for 30 min. LAMP2 is pseudo-colored in red and EGF in green, and yellow represents co-localization. Average percent co-localization values and standard deviations are given (n>25 fields of cells; P<0.005). Scale bar, 35 μm. B. Boxplot of LAMP2 co-localization with EGF as in Fig. 2. C. EGFR degradation was calculated in cells that were starved overnight, pre-treated with DMSO or AKTVIII for 45 min and CHX for the last 30 min of inhibitor treatment and stimulated with betacellulin for the indicated time points. N=2 independent experiments.