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. 2014 Jun 2;9(6):e98460. doi: 10.1371/journal.pone.0098460

Figure 2. Reactivity of pooled Ugandan serum samples and a vaccinated mouse serum pool against synthetic peptide series I.

Figure 2

(A) High, (B) medium-high, (C) medium-low, (D) low titer sera pool. Each pool consists of equal aliquots of 9–10 individual sera. The geometric mean anti-SE36 IgG titers of the individual samples are in Table S2. The patterns of reactivity for 18 individual sera are shown in Fig. S2. Serum samples were diluted 800-fold. Secondary antibody was peroxidase-conjugated goat IgG fraction to human IgG (whole molecule) (55220; Cappel ICN Pharmaceuticals Inc, Aurora, OH) diluted 1∶2000. (E) Pooled serum from five mice used at 1∶1,600. Secondary antibody was peroxidase conjugated affiniPure goat anti-mouse IgG antibody (H+L) (115-035-166; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) diluted 1∶5000. All sera were tested for ELISA at least four times. Error bars reflect standard deviation. Reactivity of malaria naïve Japanese serum and naïve mouse serum are shown in Fig. S2.