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. 2014 Jun 2;9(6):e98460. doi: 10.1371/journal.pone.0098460

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© 2014 Yagi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The reactivity of the serum samples from vaccinated (A–C) and non-vaccinated (D, E) squirrel monkeys against the different peptides in Fig. 1 and Table S1 series I. Red and green bars represent samples before challenge infection and two weeks after challenge infection, respectively. R57, R59-62 are subject codes for squirrel monkeys. Monkey serum were diluted 1∶400; secondary antibody was horseradish peroxidase-conjugated rabbit anti-human IgG antibody (A8792; Sigma-Aldrich Corp., St. Louis, MO) diluted 1∶2000. Mean values from duplicate ELISA with individual data points are shown.