Table 1.
Primers used in this study
| Name | Sequence (5′ → 3′) a | Description |
|---|---|---|
| p1 forward |
CGCCATGGATTTGAAGGTTGAATTCTAC |
For construction of pETcbd |
| p2 reverse |
CGGGATCC
AGCGGAGCCAGCGGAGCC
CACCGGGTTCTTTACCC |
|
| p3 forward |
GCGGATCCATGGGAATTAAGTTACCCACATTG |
For construction of pETcbdgla |
| p4 reverse |
CGCTCGAGTTTTTTGATTCTCTCAAATACAT |
|
| p5 forward |
CGCATATGGGAATTAAGTTACCCACATTG |
For construction of pETgla |
| p6 reverse |
GCGGATCC
AGCGGAGCCAGCGGAGCC
TTTTTTGATTCTCTCAAATACAT |
|
| p7 forward |
GCGGATCCAATTTGAAGGTTGAATTCTACAAC |
For construction of pETglacbd |
| p8 forward | CGCTCGAG CACCGGGTTCTTTACCC |
a Restriction sites are underlined, sequence encoding the linker peptide were in italics and in grey shade.