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Apparent kinetic parameters of the free fusion proteins and their immobilized counterparts
| Fusion protein | K m (mmol l -1 ) | V max (U mg -1 ) | k cat (s -1 ) |
|---|---|---|---|
| Cbd-Linker-Gla |
14.3 ± 1.6 |
71.6 ± 4.2 |
8.8 × 104 |
| Immobilized Cbd-Linker-Gla |
34.0 ± 1.6 |
344.9 ± 31.2 |
4.2 × 105 |
| Gla -Linker- Cbd |
5.7 ± 0.1 |
151.5 ± 30.3 |
1.8 × 105 |
| Immobilized Gla -Linker- Cbd | 25.0 ± 1.6 | 381.6 ± 44.2 | 4.7 × 105 |
