Formation of mixed oligomers of the wild-type HspB5 and its N-terminal (a, b) or C-terminal (c, d) chimeras. Each panel presents elution profiles of isolated wild-type HspB5 (1, dotted line), isolated fluorescent chimera (2, dashed line), the algebraic sum of these two profiles (3, dash-dotted line), and real elution profile of the mixture of the wild-type protein and its fluorescent chimera (4, solid line) preincubated either at 4 °C (a, c) or 42 °C (b, d)