Fig. 6.

Chaperone-like activity of HspB6 and its fluorescent chimeras using insulin as a model substrate. Reduction-induced aggregation of isolated insulin (1) or insulin in the presence of the wild-type HspB6 (2) or its N-terminal (3) or C-terminal (4) fluorescent chimeras. The concentration of insulin was equal to 50 μM per monomer, and the concentration of sHsp was equal to 5 μM per monomer. Data are representative of three experiments