Table 3.
Amino acid sequences of the HSP70 peptides selected for study
| No. | Amino acids (aa) | Sequence | Eluted from | In silico binding | Notes/remarks |
|---|---|---|---|---|---|
| H38 | 38-52 | TPSYVAFTDTERLIG | DR 7 binding epitope (Chicz et al. 1993) | DR 7 binding goodDR1, DR4 binding low | |
| H161 | 161-175 | AGTIAGLNVLRIINE | DR4 (Dengjel et al. 2005; Halder et al. 1997; Sanjeevi et al. 2002) | Pan HLA binders in silico | Possibly include binding site for dnaJ (Cheetham et al. 1998); presented upon autophagy |
| H167 | 167-181 | LNVLRIINEPTAAAI | |||
| H235 | 235-249 | NRMVNHFIAEFKRKH | Frameshift of epitope eluted from DR11 (Newcomb et al. 1993) | Good DR4 binder in silico | |
| H290 | 290-304 | GIDFYTSITRARFEE | DR11 (Newcomb et al. 1993) | Includes also in silico DR1, 4 and 11 binding epitopes | |
| H443 | 443-457 | YEGERAMTKDNNLLG | DR4 and/or DR4/DR53 (Dengjel et al. 2005; Friede et al. 1996) | Mainly DR4 binding epitopes in silico | Presented upon autophagy |
| H482 | 482-496 | ANGILNVSAVDKSTG | Region identified by two groups, from DR4 (Dengjel et al. 2005; Lippolis et al. 2002) | DR4 binding in silico | Presented upon autophagy |
Peptides were named with H (=human) and the position of the first amino acid of the peptide in the Hsc70 protein. Reasons for selecting the peptides are described