Table 1.
Name, sequence, and technique for polymerase chain reaction (PCR) primers used in the cloning and expression of the HSP70 gene from Pomacea canaliculata
| Primer | Sequence (5′–3′) | Technique |
|---|---|---|
| HSP70-3-1 | TGCCGACCTGTTCCGTAAGACCCTG | HSP70 3′-RACE |
| HSP70-3-2 | ACGACAGATCCACGAAGTGGTGCTG | HSP70 3′-RACE |
| M13 | GTTTTCCCAGTCACGAC | HSP70 3′-RACE |
| HSP70-5-1 | CGTCTTGGTCTCGCCCTTGTATTGC | HSP70 5′-RACE |
| HSP70-5-2 | TCGTCAAAACGCCTGCCGATCAGTC | HSP70 5′-RACE |
| HSP70-c-sf1 | GTSGAAATCATCGCSAACGACCARGG | Amplification of HSP70 core region |
| HSP70-c-sf1 | GCDGCACCATARGCWACAGCYTC | Amplification of HSP70 core region |
| Rt-hsp70-sf | GTGTGCCGACCTGTTCCGTAA | Real-time PCR |
| Rt-hsp70-sr | TGGATTTGTTCAGCTCTTTGC | Real-time PCR |
| 18S-sf | CCGTCCCTTTTGGTGACTCTG | Real-time PCR |
| 18S-sr | GGATGTGGTAGCCGTTTCTC | Real-time PCR |