Figure 1.

Schematic of protein constructs used in this work. E6N2 is a homodimeric fusion protein containing the dsRBD from human protein kinase R, the mouse IgG2a Fc fragment, and the EGFR-binding Fn3 clone, E6. D-PFO is a fusion protein with EGFR-binding Fn3 clone D and the cholesterol-dependent cytolysin, PFO. HNB-LCD is a multispecific construct described by Spangler et al.¹⁶ and contains EGFR-binding Fn3 clones B and D on the N terminus of the heavy chain and the C terminus of the light chain of cetuximab, respectively. All proteins are drawn with the N-terminus on top and the C-terminus on the bottom. dsRBD, double-stranded RNA-binding domain; PFO, perfringolysin O.