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. 2014 May 23;15(1):395. doi: 10.1186/1471-2164-15-395

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© Monleau et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Assessment of the TLDA reproducibility. The TLDA reproducibility was assessed by testing, from one RNA sample, two separate qPCR amplifications from A- the same RT and pre-amplification product (RT1-d1 vs RT1-d2: Bland-Altman) and B- separate RT and pre-amplification reactions (RT1 (d1) vs RT2: Bland-Altman). RT with 150 ng of RNA isolated from 3×10⁶ cells with Macherey-Nagel kit. Only miRNAs with Ct < 32 were considered.