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. 2014 May 23;15(1):395. doi: 10.1186/1471-2164-15-395

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© Monleau et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of miRNAs expression profiles from human PBMCs and serum. Human PBMCs (1×10⁶ cells) and serum (300 μL) samples were extracted by Macherey-Nagel (MN) and Qiagen (Q) kits. A- Number of miRNAs detected from PBMCs RNA samples using TLDA cards. B- Correlation analysis from PBMCs samples. C- Bland-Altman analysis MN versus Q from PBMCs. D- Number of miRNAs detected from serum RNA samples using TLDA cards. E- Correlation analysis from serum samples. F- Bland-Altman analysis MN versus Q from serum. TLDA data were obtained from biological triplicate for each extraction kits. Analysis using mean Ct values of triplicate. Only miRNAs with Ct < 32 were considered for PBMCs. No Ct cut-off was applied for serum miRNAs.