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. 2014 May 23;15(1):395. doi: 10.1186/1471-2164-15-395

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© Monleau et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Assessment of bias in RNA isolation using Macherey-Nagel kit from 300 or 600 μL serum. A- Bland-Altman analysis 600 versus 300 μL of serum. B- Plot of the difference in Ct values of the two conditions (x-axis) and the GC content of the miRNAs detected in these two settings (y-axis). The Pearson correlation coefficient is indicated. The GC content corresponds to the percentage of GC in the sequence of mature miRNAs. TLDA datas from biological duplicate. Analysis using mean CT values of common miRNAs, without Ct cut-off.