Table 1.
Assessment of quantity and quality of RNA isolated
| A | Macherey Nagel | Qiagen | Norgen | |
|---|---|---|---|---|
| PBMC | n | 8 | 10 | 10 |
| Nanodrop | Total RNA: Mean quantity (ug/1×106 cells or ml serum) (SD) | 1.03 (0.22) | 0.97 (0.14) | 1.93 (0.69) |
| Ratio OD (260 nm/280 nm) (SD) | 2.04 (0.07) | 1.75 (0.06) | 1.91 (0.07) | |
| Agilent Nano 6000 Chip | Total RNA: Mean Quantity (ug/1×106 cells or ml serum) (SD) | 1.19 (0.33) | 0.96 (0.30) | 0.98 (0.50) |
| RIN mean (SD) | 8.84 (0.34) | 8.73 (0.41) | 7.33 (2.93) | |
| B | Macherey Nagel | Qiagen | Norgen | |
| Serum | n | 10 | 10 | 10 |
| Nanodrop | Total RNA: Mean quantity (ug/1×106 cells or ml serum) (SD) | 0.91 (0.46) | 0.89 (0.40) | 0.99 (0.34) |
| Ratio OD (260 nm/280 nm) (SD) | 1.24 (0.11) | 1.40 (0.13) | 1.27 (0.52) |
The quantity and quality of RNA isolated from (A) PBMCs and (B) serum using three RNA extraction kits (Macherey-Nagel, Qiagen, Norgen) were evaluated by Nanodrop, Agilent nano 6000 (PBMCs only) and Agilent small RNA chips.