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. 2014 May 28;5:253. doi: 10.3389/fmicb.2014.00253

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Copyright © 2014 Alfadhli and Barklis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Competition of PI(4,5)P2 liposomes for matrix protein RNA binding sites. Experiments performed by Alfadhli et al. (2011) have shown that nickel-nitrilotriacetic acid (Ni-NTA) beads coated with the myristoylated HIV-1 matrix protein (MyrMA) bind fluorescently tagged Sel25 RNA (MyrMA + RNA). Addition of liposomes composed of 80% dioleoylphosphatidyl choline + 20% cholesterol (plus PC) during binding reactions did not diminish MA–RNA binding, whereas addition of an equal amount of liposomes composed of 70% PC, 10% PI(4,5)P2, 20% cholesterol (plus PIP2) significantly reduced MA–RNA binding. These and other experiments indicate that the affinity of HIV-1 MA for RNA is less than that for membranes containing PI(4,5)P2 but greater than that for membranes without PI(4,5)P2.