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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Allergy Clin Immunol. 2013 May 16;132(2):437–445. doi: 10.1016/j.jaci.2013.03.024

Figure 5. MEK/ERK up-regulation is downstream of the ROS production induced by anti-Siglec-8/IL-5 co-stimulation.

Figure 5

Eosinophils were pre-incubated with or without inhibitors and then cultured in media containing IL-5 and anti-Siglec-8. The lower panel shows Annexin V staining of cells from the same experiment at the 24-hour time point. In A, cells were stained with DHR and ROS level analyzed with flow cytometry (representative of 3 experiments). In B, Western blot of lysates from cells harvested after 10 minutes of stimulation is shown (representative of 3 experiments). Ratio of densitometric value for band of phospho-ERK to that of total ERK in each lane is displayed. *p <0.01 and §p <0.01, compared to % Annexin V+ 7AAD+ and % Annexin V+ 7AAD−, respectively, in IL-5 + anti-Siglec-8 treated cells without inhibitors.