Figure 4.

Mutually exclusive interactions of MYST1 with Sirt1 or AR in PCa cells. A and B, Endogenous MYST1 interacted with Sirt1 in PC3 cells but not in PC3-AR and LNCaP cells wherein MYST1 interacts with AR. C, Endogenous AR interacted with the full-length and the 157–458 region of MYST1. D, Induction of PSA luciferase activity in LNCaP cells by increasing amounts of MYST1-expressing plasmid upon treatment with 10 nM R1881 treatment confirms the role of MYST1 as a coactivator of AR. E, Immunohistochemical analysis with anti-MYST1 antibodies of formalin-fixed human prostate cancer tissues (n = 5) revealed that MYST1 was nuclear in the benign prostate epithelium adjacent to PCa cells, in which MYST1 was cytoplasmic as well as nuclear. Original magnification, ×200.