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. 2014 Apr 8;28(6):899–911. doi: 10.1210/me.2014-1051

Figure 5.

Figure 5.

In vitro assays demonstrate WT binding to the Igf1 ERE motif. A, RT-PCR. Analysis and statistical analyses were performed as described for Figure 2A. B, Luciferase reporter gene activity. Igf1 ERE-luc analysis and statistical analyses were performed as described for Figure 3E. C, ChIP-PCR. ChIP-PCR analysis and statistical analyses were performed as described for Figure 2C. D, In vitro DNA-binding assay. Biotinylated Igf1 ERE-binding to WT, KIKO, or EAAE ERα-DNA and statistical analysis were performed as described for Figure 3G. Nonbiotinylated (unlabeled) DNA positive control, Igf1 ERE; negative control, Igf1 NRE.