Fig. 4.

Quantification of mast cells and mast cell proteases in the jejunum of WT and GPR65-deficient mice. A: flow cytometric examination of EGFP in the jejunal mast cells from Gpr65^+/+ and Gpr65^−/− (EGFP knock-in) mice following OVA challenges. B: the mast cells within jejunal lamina propria and crypt areas were identified as chloroacetate esterase (CAE) activity-positive (red) cells and quantified by an observer blinded to treatment and genotype. C: representative images of CAE staining on the jejunum from saline-challenged WT mice (a), OVA-challenged WT mice (b), saline-challenged GPR65-deficient mice (c), and OVA-challenged GPR65-deficient mice (d). D–F: the transcript levels of murine Mcpt1 (D), Mcpt2 (E), and Mcpt4 (F) relative to the housekeeping gene Actb in the jejunum of WT and GPR65-deficient mice were determined by real-time RT-PCR. All data are a representative of 4 experiments and expressed as means ± SD (n = 4–6 mice/saline group, n = 7–9 mice/OVA group). **P < 0.01; ns, no significant difference.