Fig. 5.

Inhibition of MMP activation attenuates C12-HSL-induced decrease in occludin and tricellulin expression. Cells were pretreated with 25 μM ARP100 (ARP), 25 μM NNGH, or 25 μM GM1489 (GM) for 1 h prior to incubation with 200 μM C12-HSL for 4 h. Controls were either untreated (CTL) or incubated with vehicle (0.4% DMSO, Veh). Expression levels of occludin (A) and tricellulin (B) were assessed by immunoblotting. Representative blots of 3 independent experiments. Densitometric analysis of the specific bands was performed with the Image J program. Values are means ± SD. *P < 0.05, **P < 0.01 vs. CTL, †P < 0.05, ††P < 0.01 vs. C12-HSL-treated cells.