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. 2014 Mar 31;28(6):647–658. doi: 10.1016/j.devcel.2014.01.022

Figure 1.

Figure 1

PI3K-C2α Is Enriched at the Pericentriolar Recycling Compartment around the Ciliary Base

(A) Immunofluorescence of quiescent MEFs to detect PI3K-C2α-GFP (green), acetylated α-tubulin (red), and DNA (blue), showing that transfected PI3K-C2α accumulates perinuclearly. Bar = 400 nm.

(B) Staining of the centrioles (γ-tubulin, blue), and the primary cilium (acetylated α-tubulin, red) show that endogenous PI3K-C2α (green) localizes around the ciliary base. bar = 200 nm.

(C) Costaining of PI3K-C2α-GFP (green) with markers of recycling endosomes Rab11 (red, upper panes) and transferrin receptor (TfR, red, lower panels) shows high degree of colocalization. Bar = 400 nm.

(D) Cell fractionation showing that PI3K-C2α is absent from late endosomes (LE), while it is enriched in the early endosomal (EE) and cytosol/heavy membrane fraction (Cyt+HM), similar to what observed for Rab11. PNS, postnuclear supernatant.