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qRT-PCR verification of RNA-seq values for select mRNAs and representative immunohistochemical staining for the total macrophage marker CD68 in positive control (spleen) and in OMAT from lean and obese Ossabaw swine. There were no significant differences between groups for these genes or for CD68 positive staining in OMAT. Note the brown staining of CD68 in the spleen positive control is not present in the OMAT slides.
