Fig. 2.

Effect of cytomix plus hypoxia on the release of soluble mediators by MSCs. The MSC were cultured in DMEM in the absence or presence of cytomix (20 ng/ml) plus hypoxia (3% O₂). After 12 h, the media of nonexposed cells (MSC-M) or the media of cells exposed to cytomix (20 ng/ml) plus hypoxia (3% O₂; HCYT-MSC-M) were used for the determination of IL-1 receptor antagonist (IL-1ra), PGE₂, and keratinocyte growth factor (KGF) concentrations. The values represent the means ± SD of 4–6 experiments in each group. *P < 0.01 by unpaired Student's t-test.