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. 2014 Apr 4;306(11):L1006–L1015. doi: 10.1152/ajplung.00015.2014

Fig. 7.

Fig. 7.

Effect of MMP-9 suppression on transcriptional activity of Smad3. A: effect of MMP-9 depletion on Smad3 reporter activity. HLF-1 cells transfected with MMP-9 siRNA or control siRNA were cotransfected with CAGA12-Luc reporter plasmid together with pRL-TK. The cells were then treated with or without TGF-β1 for 30 min. Vertical axis, Smad3 reporter activity; horizontal axis, cells were treated with or without TGF-β1 (100 pM); open bars, cells transfected with control siRNA and reporter plasmid; hatched bars, cells transfected with MMP-9 siRNA and reporter plasmid. Data are shown as means ± SE. *P < 0.05. B: effect of MMP-9 depletion on fibronection production by HFL-1 cells. After collagen gel contraction assay, the media were harvested on day 2 and used for fibronection quantification by ELISA. Vertical axis, fibronection concentration (ng/ml); horizontal axis, culture medium with or without TGF-β1 (100 pM); open bars, control siRNA-transfected cells; hatched bars, MMP-9 siRNA-transfected cells. *P < 0.05.