Fig. 3.
Blockade of large conductance Ca2+-activated K+ channels (BKCa) with paxilline abolishes STOCs in SEA SMCs. A and B: representative gap-free recordings of STOCs in a SMC from Young held at +30 mV. A: exposure to 0.1% DMSO (vehicle for paxilline) increased STOC amplitude and frequency as shown. B: subsequent exposure to paxilline (1 μM) in 0.1% DMSO abolished STOCs. C–F: data are mean ± SE amplitude (C and D) and frequency (E and F) of STOCs recorded in SMCs from Young (C and E; n = 6 cells from 3 mice) or Old (D and F; n = 6 cells from 3 mice) before application of DMSO (Control), after application of 0.1% DMSO, and after application of 1 μM paxilline in 0.1% DMSO. *P < 0.05 compared with Control.