Fig. 3.

Recovery of spiked recombinant rat VEGF (rrVEGF) in rat plasma using the rat and mouse VEGF ELISAs. Percent recovery of a 980 pg/ml spike of rrVEGF is shown for the same samples run on the rat and mouse ELISAs. Percent recovery differed significantly between the rat and mouse ELISAs (P = 0.04). A single aliquot from each rat was used to prepare neat and spiked plasma samples, which were then run on the rat and mouse ELISAs at the same time. Spiked plasma was prepared in a single tube; then loaded directly onto the rat ELISA or pipetted into a second tube for dilution with assay diluent before loading onto the mouse ELISA. To reduce the complexity of the figure, lines were not used to show pairing.