Table 1.
VEGF Concentrations in late pregnant Sprague-Dawley rats in the control group
| Study | Day of Gestation | Rat VEGF, pg/ml | ELISA§ | Sample |
|---|---|---|---|---|
| Gilbert et al., 2007 (15) | 19 | 833 ± 37‡ | Mouse | EDTA plasma |
| Bridges et al., 2009 (8) | 18 | 780 ± 48† | Mouse | EDTA plasma |
| Gilbert et al., 2010 (18) | 19 | 1,404 ± 90‡ | Mouse | EDTA plasma |
| George et al., 2011 (10) | 19 | 1,030 ± 88† | Mouse | Plasma |
| George et al., 2011 (12) | 19 | 527 ± 86† | Mouse | Plasma |
| Banek et al., 2012 (4) | 19 | 795 ± 72† | Mouse | Plasma |
| Gilbert et al., 2012 (16) | 19 | 850 ± 50‡ | Mouse | EDTA plasma |
| Gilbert et al., 2012 (17) | 19 | 830 ± 70‡ | Mouse | EDTA plasma |
| Banek et al., 2013 (5) | 19 | 915 ± 65‡ | Mouse | Plasma |
| Bauer et al., 2013 (7) | 19 | 925 ± 45‡ | Mouse | EDTA plasma |
| George et al., 2013 (13) | 19 | 287 ± 23‡ | Mouse | Plasma |
| George et al., 2013 (14) | 19 | 1,017 ± 95† | Mouse | Plasma |
| Lillegard et al., 2013 (21) | 19 | 1,004 ± 100‡ | Mouse | EDTA plasma |
| Agunanne et al., 2010 (2) | 17–20 | 29 ± 2* | Rat | Plasma |
| Ramesar et al., 2011 (24) | 20 | 8 ± 1† | Rat | Heparin plasma |
| Bahtiyar et al., 2007 (3) | 21 | 579 ± 14† | Rat | Serum# |
Values are means ± SD.
Mean ± SE or
mean and SE estimated from a figure using Plot Digitizer 2.6.3.
Confirmed with study authors.
VEGF concentrations in serum are higher than those in plasma as VEGF is released from platelets and other blood components during clotting (19). Two additional studies that used the mouse ELISA to measure vascular endothelial growth factor (VEGF) in rat samples were not listed. One study measured VEGF in media from rat placental villous explants (11). The second compared plasma VEGF in lean wild-type Wistar Hannover rats and obese melanocortin-4-receptor heterozygous rats on day 19 of gestation (25). The mean plasma VEGF concentrations in the lean rats was approximately 2,865 pg/ml (25).