Fig. 4.

Expression of autophagic markers in myoblasts. A: there was no change in the mRNA expression of two apoptotic genes, Beclin-1 and ATG5 at d5 of proliferation and differentiation between the CBA and SUC groups. B: there was no significant change in the labeling index for LC3B, an autophagosomal protein, during d5 of proliferation and differentiation between the two groups. C: representative images of LC3B immunostaining in myoblasts of SUC and CBA groups. Arrows indicate LC3B immunostaining. Scale bar = 10 μm. Data are expressed as means ± SE.