CD8+ T cells from RAG1−/− OT-1 (black) or RAG1−/− OT-1 Ikzf1+/− (grey) mice were sorted by naïve (CD62LhiCD44lo) phenotype, stimulated with plate bound anti-CD3/CD28 (A) with exogenous IL-2, IL-12 or anti-IL-2 for 48 hours, and analyzed for expression of CD25 and IFNγ (MFI is depicted in upper right quadrant), or for expression of Eomes and T-bet (B) by flow cytometry. Two biological replicates are shown for each group, and data are representative of two independent experiments.