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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Immunol. 2014 Apr 28;192(11):5118–5129. doi: 10.4049/jimmunol.1301992

Fig. 3. CD8+ T cells hemizygous for the Ikzf1 gene exhibit enhanced effector differentiation.

Fig. 3

CD8+ T cells from RAG1−/− OT-1 (black) or RAG1−/− OT-1 Ikzf1+/− (grey) mice were sorted by naïve (CD62LhiCD44lo) phenotype, stimulated with plate bound anti-CD3/CD28 (A) with exogenous IL-2, IL-12 or anti-IL-2 for 48 hours, and analyzed for expression of CD25 and IFNγ (MFI is depicted in upper right quadrant), or for expression of Eomes and T-bet (B) by flow cytometry. Two biological replicates are shown for each group, and data are representative of two independent experiments.