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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Immunol. 2014 Apr 28;192(11):5118–5129. doi: 10.4049/jimmunol.1301992

Fig. 4. Ikzf1+/− CD8+ T cells produce autocrine IL-2.

Fig. 4

Naïve-enriched CD8+ T cells from Ikzf1+/+ or Ikzf1+/− B6 or RAG1−/− OT-I mice were stimulated as in Fig. 2, and culture supernatants were assayed for IL-2 production by ELISA at the indicated time points (A) or by intracellular staining at 6 and 48 hours (B). Plots in B are gated on CD69+ cells. In C, naïve-enriched Ikzf1+/+ or Ikzf1+/− OT-I CD8+ T cells were stimulated for 24 hours with either a fixed concentration of plate-bound anti-CD28 and a titration of anti-CD3 (left panel), or a fixed concentration of anti-CD3 and a titration of anti-CD28 (right panel). Values plotted in C represent the mean+/−SEM of duplicate cultures and are representative of 2 independent experiments.