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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Immunol. 2014 Apr 28;192(11):5118–5129. doi: 10.4049/jimmunol.1301992

Fig. 5. IL-2 produced by Ikzf1+/− CD8+ T cells can act in a paracrine fashion to induce differentiation of wild-type CD8+ T cells.

Fig. 5

Naïve-enriched CD8+ T cells from Ikzf1+/+ (CD45.1+) or Ikzf1+/− (CD45.2+) mice were stimulated in individual cultures as in Fig. 1 (A), or in co-cultures at a 1:1 ratio for 48 hours in the absence (B) or presence (C) of anti-IL-2 neutralizing antibody. Expression of CD25 and IFN-γ were assessed by flow cytometric analysis, and numbers represent %IFN-γ+CD25+ cells. CD25 expression in Ikzf1+/+ (black) and Ikzf1+/− (gray) cultures is also illustrated in histogram overlays (bottom panels in A–C). Supernatants from these cultures were assessed for IL-2 and IFN-γ production by ELISA (D, E and F). Data are representative of 3 independent experiments.