SAL-SAL |
Saline(1ml/kg)-Saline(1ml/kg) |
Nociceptive behavioral test on days -4, 0, 1, 3, 5, 7, and 14; Morphine dose-response test on Day 7 and 14 |
SAL-MID |
Saline(1ml/kg)-midazolam (2mg/kg) |
Nociceptive behavioral test on days -4, 0, 1, 3, 5, 7, and 14; Morphine dose-response test on Day 7 and 14 |
MOR-SAL |
Morphine(10mg/kg)-Saline(1ml/kg) |
Nociceptive behavioral test on days -4, 0, 1, 3, 5, 7, and 14; Morphine dose-response test on Day 7 and 14 |
MOR-MID |
Morphine(10mg/kg)-midazolam (2mg/kg) |
Nociceptive behavioral test on days -4, 0, 1, 3, 5, 7, and 14; Morphine dose-response test on Day 7 and 14 |
SAL-SAL-Vehicle |
Saline(1ml/kg)-Saline(1ml/k g) +3%DMSO(5μl) |
Morphine dose-response test on Day 14 |
SAL-SAL-Chelerythrine |
Saline(1ml/kg)-Saline(1ml/k g)+ chelerythrine chloride (10 nmol) |
Morphine dose-response test on Day 14 |
MOR-MID-Vehicle |
Morphine(10mg/kg)-midazolam (2mg/kg)+ 3%DMSO(5μl) |
Morphine dose-response test on Day 14 |
MOR-MID-Chelerythrine |
Morphine(10mg/kg)-midazolam (2mg/kg)+ chelerythrine chloride (10 nmol) |
Morphine dose-response test on Day 14 |