Figure 3. Reduction of spontaneous inward current by the TRPM4 channel inhibitor 9-phenanthrol in the presence of the muscarinic receptor agonist carbachol in freshly-isolated rat DSM cells. (A) A representative recording illustrating the inhibitory effect of 9-phenanthrol (30 μM) on spontaneous inward current recorded in the presence of carbachol (1 µM) at −70 mV in a single DSM cell using the perforated whole cell patch-clamp technique. (B) Summary data for the inhibitory effect of 9-phenanthrol on TRPM4 spontaneous inward current in the presence of carbachol (1 µM), presented as NPo before (control) and after application of 30 μM 9-phenanthrol (n = 11, N = 6; *p < 0.05).